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resuspension buffer  (Qiagen)


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    Structured Review

    Qiagen resuspension buffer
    Resuspension Buffer, supplied by Qiagen, used in various techniques. Bioz Stars score: 94/100, based on 47 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/mircury+kit/miRCURY+Exosome+Cell%2FUrine%2FCSF+Kit/pmc13065346-170-13-15
    Average 94 stars, based on 47 article reviews
    resuspension buffer - by Bioz Stars, 2026-10
    94/100 stars

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    Related Articles

    Lysis:

    Article Title: Preclinical spheroid models identify BMX as a therapeutic target for metastatic MYCN nonamplified neuroblastoma
    Article Snippet: For tumor samples, dissociation was performed following gentleMACS Dissociator kit instructions for soft tumors (Miltenyi Biotech). .. Total RNA was extracted with miRCURY kit (Exiqon) using Lysis buffer + B-mercaptoethanol. .. Minimum 100 ng used for preparing sequencing libraries using the TruSeq mRNA stranded kit (Illumina) and sequencing was performed on a Hi-Seq Illumina Genome Analyzer (100 bp single-read).

    Article Title: Preclinical spheroid models identify BMX as a therapeutic target for metastatic MYCN nonamplified neuroblastoma.
    Article Snippet: For tumor samples, dissociation was performed following gentleMACS Dissociator kit instructions for soft tumors (Miltenyi Biotech). .. Total RNA was extracted with miRCURY kit (Exiqon) using Lysis buffer + B-mercaptoethanol. .. Minimum 100 ng used for preparing sequencing libraries using the TruSeq mRNA stranded kit (Illumina) and sequencing was performed on a Hi-Seq Illumina Genome Analyzer (100 bp single-read).

    Next-Generation Sequencing:

    Article Title: Current Evidence Supporting the Role of miRNA as a Biomarker for Lung Cancer Diagnosis Through Exhaled Breath Condensate Collection: A Narrative Review
    Article Snippet: Mithcell et al. (2024) [ ] , RTube (Respiratory Research Inc., Austin, TX, U.S.), 10 min tidal breathing, 1.5–2 mL , Differential ultracentrifugation + EV-CATCHER , miRNeasy Serum/Plasma Kit (Qiagen), 100 μL EVs , Custom PAGE-purified small RNA library , Illumina HiSeq 2500 , RNAworld trimming, DESeq2/edgeR via Bioconductor. .. Stachowiak et al. (2020) [ ] , TurboDeccs (Medivac Srl, Parma, Italy), −5 °C, 3 mL collected , Exosomes from EBC using miRCURY kit (Qiagen) , RNeasy mini kit + MinElute columns (Qiagen) , TruSeq Small RNA Library Kit (Qiagen) , Illumina MiniSeq , BaseSpace for NGS data; qPCR validation (miR-486-5p, miR-223, etc.). .. Exhaled Breath Condensate , - Completely non-invasive - High patient compliance - Reflects airway-specific processes - Suitable for repeated sampling , - Low miRNA concentration - High dilution (>99.9% water) - Lack of standardization - Contamination risk (oral/upper airway).

    Real-time Polymerase Chain Reaction:

    Article Title: Current Evidence Supporting the Role of miRNA as a Biomarker for Lung Cancer Diagnosis Through Exhaled Breath Condensate Collection: A Narrative Review
    Article Snippet: Mithcell et al. (2024) [ ] , RTube (Respiratory Research Inc., Austin, TX, U.S.), 10 min tidal breathing, 1.5–2 mL , Differential ultracentrifugation + EV-CATCHER , miRNeasy Serum/Plasma Kit (Qiagen), 100 μL EVs , Custom PAGE-purified small RNA library , Illumina HiSeq 2500 , RNAworld trimming, DESeq2/edgeR via Bioconductor. .. Stachowiak et al. (2020) [ ] , TurboDeccs (Medivac Srl, Parma, Italy), −5 °C, 3 mL collected , Exosomes from EBC using miRCURY kit (Qiagen) , RNeasy mini kit + MinElute columns (Qiagen) , TruSeq Small RNA Library Kit (Qiagen) , Illumina MiniSeq , BaseSpace for NGS data; qPCR validation (miR-486-5p, miR-223, etc.). .. Exhaled Breath Condensate , - Completely non-invasive - High patient compliance - Reflects airway-specific processes - Suitable for repeated sampling , - Low miRNA concentration - High dilution (>99.9% water) - Lack of standardization - Contamination risk (oral/upper airway).

    Biomarker Discovery:

    Article Title: Current Evidence Supporting the Role of miRNA as a Biomarker for Lung Cancer Diagnosis Through Exhaled Breath Condensate Collection: A Narrative Review
    Article Snippet: Mithcell et al. (2024) [ ] , RTube (Respiratory Research Inc., Austin, TX, U.S.), 10 min tidal breathing, 1.5–2 mL , Differential ultracentrifugation + EV-CATCHER , miRNeasy Serum/Plasma Kit (Qiagen), 100 μL EVs , Custom PAGE-purified small RNA library , Illumina HiSeq 2500 , RNAworld trimming, DESeq2/edgeR via Bioconductor. .. Stachowiak et al. (2020) [ ] , TurboDeccs (Medivac Srl, Parma, Italy), −5 °C, 3 mL collected , Exosomes from EBC using miRCURY kit (Qiagen) , RNeasy mini kit + MinElute columns (Qiagen) , TruSeq Small RNA Library Kit (Qiagen) , Illumina MiniSeq , BaseSpace for NGS data; qPCR validation (miR-486-5p, miR-223, etc.). .. Exhaled Breath Condensate , - Completely non-invasive - High patient compliance - Reflects airway-specific processes - Suitable for repeated sampling , - Low miRNA concentration - High dilution (>99.9% water) - Lack of standardization - Contamination risk (oral/upper airway).

    other:

    Article Title: Extracellular vesicles secreted by human uterine stromal cells regulate decidualization, angiogenesis, and trophoblast differentiation.
    Article Snippet: EVs were isolated by the miRCURY (Qiagen) kit, and the concentration of resuspended EVs was determined by MRPS (Spectradyne, LLC).

    Article Title: Phthalates Impair Estrogenic Regulation of HIF2α and Extracellular Vesicle Secretion by Human Endometrial Stromal Cells.
    Article Snippet: Representative data are shown as mean fold change ± SEM (n = 2 independent experiments). (F) EVs were isolated from conditioned media of an equal number of differentiating HESCs treated with vehicle, DEHP, or MEHP using the miRCURY (Qiagen) kit and analyzed by MRPS (Spectradyne, LLC).

    Article Title: Phthalates Impair Estrogenic Regulation of HIF2α and Extracellular Vesicle Secretion by Human Endometrial Stromal Cells.
    Article Snippet: After 72 hours of differentiation in hypoxic conditions, while exposed to 1 μg/mL DEHP or MEHP or vehicle, we collected the conditioned media and isolated EVs using a miRCURY (Qiagen) kit.

    Article Title: Phthalates Impair Estrogenic Regulation of HIF2α and Extracellular Vesicle Secretion by Human Endometrial Stromal Cells.
    Article Snippet: Data are shown as mean fold change ± SEM (n = 3-4 independent experiments), *P < .05, relative to control siRNA treatment. (D) EVs were isolated from conditioned media using the miRCURY (Qiagen) kit and analyzed by MRPS (Spectradyne, LLC).

    Isolation:

    Article Title: miRNA 548a-3p as biomarker of NEDA-3 at 2 years in multiple sclerosis patients treated with fingolimod
    Article Snippet: .. RNA was isolated using the miRcury kit (Exiqon, Woburn, MA) and converted to complementary DNA using a cDNA synthesis kit from Exiqon following the manufacturer’s instructions. ..

    cDNA Synthesis:

    Article Title: miRNA 548a-3p as biomarker of NEDA-3 at 2 years in multiple sclerosis patients treated with fingolimod
    Article Snippet: .. RNA was isolated using the miRcury kit (Exiqon, Woburn, MA) and converted to complementary DNA using a cDNA synthesis kit from Exiqon following the manufacturer’s instructions. ..



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